Entry 21 of 630 from Query 38   * Names only * * Complete entries * CABRI_Cell_Lines_View CABRI_Std_View

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CABRI:DSMZ_MUTZ Accession_number ACC 587 Cell_line_name 7926 Brief_description confirmed as mouse by species PCR murine radiation-induced acute myeloid leukemia (AML) cell line derived from splenocytes of the C3H inbred mouse strain in 1997; cell line was described to be radio-sensitive Description mouse acute myeloid leukemia murine radiation-induced acute myeloid leukemia (AML) cell line derived from splenocytes of the C3H inbred mouse strain in 1997; cell line was described to be radio-sensitive confirmed as mouse by species PCR Depositor Dr. P. Finnon, NRPB, Oxon, UK Morphology mostly round cells growing in clumps or singly in suspesion Culture_conditions 80% RPMI 1640 + 20% FBS + (10 ng/ml mIL-3) split saturated culture 1:2 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; cells are very difficult to culture, mIL-3 supports growth of the cells, cells grow best in 12- or 24-well-plates; after thawing, for the first 7-10 days there is virtually no cell growth or cells grow very slowly, viability may be initially very low, not exceeding 80-85% at 37C with 5% CO2 maximum density at about 1-1.2 x 106 cells/ml Viruses PCR: SMRV - Properties Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule Doubling_time ca. 50-80 hours Mycoplasma negative in microbiological culture, PCR assays Bibliographic_reference Finnon et al., Int. J. Radiat. Biol., 77: 43 (2001) [PMID: 11747540 ]

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